In this article, we will discuss Amsacrine (Experimental Notes-5). So, let’s get started.
These oxidation products were about 100 times more cytotoxic to cells than amsacrine in vitro, while the principal conjugation product in rats, the 5′-glutathione conjugate, was inactive (Shoemaker et al., 1984). The same conjugation products were reported after exposure of Chinese hamster fibroblasts to amsacrine or its methanesulfonyl oxidation product in culture. The rate
of glutathione conjugate formation during exposure to the oxidation product in cultured cells was rapid, whereas formation after exposure to amsacrine was slow, suggesting a low rate of oxidation of amsacrine to its oxidation products, with subsequent conjugation formation in this system (Robbie et al., 1990)