Immunophenotyping in Acute Leukemia

In this article, we will discuss the Immunophenotyping in Acute Leukemia. So, let’s get started.

Immunophenotyping

The recent development of monoclonal antibodies as well as advances in flow cytometry has made immunophenotyping readily available. It is useful in:

  • Definite lineage (B cell versus T cell) and stages of differentiation of ALL and identifying characteristic features of AML
  • Aiding in lineage determination of acute leukemias that are morphologically undifferentiated
  • In differentiating acute leukemias from other non-hematological disorders
  • Recognising mixed lineage/biphenotypic acute leukemia (ALL with myeloid markers or AML with lymphoid markers)

Immunophenotyping in acute leukemias

  • Subtype
  • ALL
  • T-cell
  • B-cell
  • Pre-B
  • CALLA
  • AML
  • M1, M2, M3
  • M4, M5
  • M6
  • M7
  • Flow cytometry
  • CD4 (helper)/CD8 (suppressor)
  • CD19(B4)/CD20(B1)/CD22(surface)
  • CD9
  • CD10
  • CD13 or CD33
  • CD11 or 13 or 14 or 15 or CD33
  • Glycoprotein, spectrin
  • CD41 (glycoprotein IIb/IIa)/CD43
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